The use of cancer biomarkers to diagnose, assess prognosis, and select a suitable treatment has become possible due to the development of inexpensive and user-friendly molecular analysis tools such as polymerase chain reaction (PCR). Maintaining the integrity of these biomarkers during processing and storage is therefore of paramount importance for accurate diagnosis and prognosis of many diseases, including cancers. To safely store samples for research or clinical uses, freezing procedures using equipment are used to arrest the degradation of cancer biomarkers. However, the stability of these molecules depends upon long-term storage conditions.

This paper aims to demonstrate the necessity of ultra-low temperature storage in maintaining the stability of important cancer biomarkers in fluid, cell, and tissue biospecimens.